![]() Received: JAccepted: JPublished: August 10, 2017Ĭopyright: © 2017 Hemmatian et al. Roeder, University of Notre Dame, UNITED STATES (2017) Accuracy and reproducibility of mouse cortical bone microporosity as quantified by desktop microcomputed tomography. Thus, μCT might be an important tool to improve our understanding of the physiological and biomechanical significance of these cannular and lacunar structure in cortical bone.Ĭitation: Hemmatian H, Laurent MR, Ghazanfari S, Vanderschueren D, Bakker AD, Klein-Nulend J, et al. In conclusion, desktop μCT is a valuable tool to quantify the 3D characteristics of bone vascular canals as well as lacunae which can be applied to intact murine bones with high accuracy and reproducibility. Intraclass correlation coefficient (ICC) showed a high reliability of the measurements, ranging from 0.998–1.000 for cortical parameters, 0.973–0.999 for vascular canal parameters and 0.755–0.991 for lacuna parameters. Reproducibility was high, with precision errors (PE) of 1.57–4.69% for lacuna parameters, and of 1.01–9.45% for vascular canal parameters. Whereas the two techniques showed no significant differences for these parameters, the mean lacuna sphericity acquired from μCT was significantly higher than CLSM (p = 0.01). An excellent correlation between μCT and CLSM was observed for both mean lacuna volume (r = 0.98, p = 0.002) and for mean lacuna orientation (r = 0.93, p = 0.02). Three repeated measurements were made on different days. The reproducibility of μCT-derived cortical bone microstructural indices was examined in 10 fibulae of C57Bl/6J male mice at a nominal resolution of 700 nanometer. The accuracy of measurements was evaluated in five murine fibula using confocal laser scanning microscopy (CLSM) in conjunction with Fluorescein isothiocyanate (FITC) staining as the reference method. In this study, we explored the accuracy and reproducibility of nondestructive desktop μCT for 3D visualization and subsequent morphometric analysis of mouse cortical bone microporosity including the vascular canal network and osteocyte lacunae. Bone's microporosity plays important roles in bone biology and bone mechanical quality. ![]()
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